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Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
To fully exploit the microbial genome resources, a high-throughput experimental platform is needed to associate genes with phenotypes at the genome level. We present here a novel method that enables investigation of the cellular consequences of repressing individual transcripts based on the CRISPR i...
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| Pubblicato in: | Nat Commun |
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| Autori principali: | , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Nature Publishing Group UK
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6018678/ https://ncbi.nlm.nih.gov/pubmed/29946130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-04899-x |
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