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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Multiple enhancers often regulate a given gene, yet for most genes, it remains unclear which enhancers are necessary for gene expression, and how these enhancers combine to produce a transcriptional response. As millions of enhancers have been identified, high-throughput tools are needed to determin...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Main Authors: Carleton, Julia B., Berrett, Kristofer C., Gertz, Jason
Formato: Artigo
Idioma:Inglês
Publicado: MyJove Corporation 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101477/
https://ncbi.nlm.nih.gov/pubmed/29912188
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57883
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