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Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Single molecule fluorescence in situ hybridization (smFISH) is a powerful technique to study gene expression in single cells due to its ability to detect and count individual RNA molecules. Complementary to deep sequencing-based methods, smFISH provides information about the cell-to-cell variation i...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Chen, Jingxun, McSwiggen, David, Ünal, Elçin
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101419/
https://ncbi.nlm.nih.gov/pubmed/29889208
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57774
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