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Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Single molecule fluorescence in situ hybridization (smFISH) is a powerful technique to study gene expression in single cells due to its ability to detect and count individual RNA molecules. Complementary to deep sequencing-based methods, smFISH provides information about the cell-to-cell variation i...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Chen, Jingxun, McSwiggen, David, Ünal, Elçin
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2018
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101419/
https://ncbi.nlm.nih.gov/pubmed/29889208
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57774
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