De Novo Generation of Somatic Stem Cells by YAP/TAZ

Here we present protocols to isolate primary differentiated cells and turn them into stem/progenitor cells (SCs) of the same lineage by transient expression of the transcription factor YAP. With this method, luminal differentiated (LD) cells of the mouse mammary gland are converted into cells that e...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Panciera, Tito, Azzolin, Luca, Di Biagio, Daniele, Totaro, Antonio, Cordenonsi, Michelangelo, Piccolo, Stefano
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2018
Subjects:
Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101120/
https://ncbi.nlm.nih.gov/pubmed/29782008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57462
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