Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Fluorescent proteins are fundamental tools for the life sciences, in particular for fluorescence microscopy of living cells. While wild-type and engineered variants of the green fluorescent protein from Aequorea victoria (avGFP) as well as homologs from other species already cover large parts of the...

Full description

Saved in:
Bibliographic Details
Published in:J Vis Exp
Main Authors: Baumann, Tobias, Schmitt, Franz-Josef, Pelzer, Almut, Spiering, Vivian Jeanette, Freiherr von Sass, Georg Johannes, Friedrich, Thomas, Budisa, Nediljko
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2018
Subjects:
Bioengineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6100899/
https://ncbi.nlm.nih.gov/pubmed/29757279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57017
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items