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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Fluorescent proteins are fundamental tools for the life sciences, in particular for fluorescence microscopy of living cells. While wild-type and engineered variants of the green fluorescent protein from Aequorea victoria (avGFP) as well as homologs from other species already cover large parts of the...

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Podrobná bibliografie
Vydáno v:J Vis Exp
Hlavní autoři: Baumann, Tobias, Schmitt, Franz-Josef, Pelzer, Almut, Spiering, Vivian Jeanette, Freiherr von Sass, Georg Johannes, Friedrich, Thomas, Budisa, Nediljko
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6100899/
https://ncbi.nlm.nih.gov/pubmed/29757279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57017
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