Efficient analysis of mammalian polysomes in cells and tissues using Ribo Mega-SEC

We describe Ribo Mega-SEC, a powerful approach for the separation and biochemical analysis of mammalian polysomes and ribosomal subunits using Size Exclusion Chromatography and uHPLC. Using extracts from either cells, or tissues, polysomes can be separated within 15 min from sample injection to frac...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Yoshikawa, Harunori, Larance, Mark, Harney, Dylan J, Sundaramoorthy, Ramasubramanian, Ly, Tony, Owen-Hughes, Tom, Lamond, Angus I
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2018
Assuntos:
Biochemistry and Chemical Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6086667/
https://ncbi.nlm.nih.gov/pubmed/30095066
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.36530
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