Real-time tracking of complex ubiquitination cascades using a fluorescent confocal on-bead assay

BACKGROUND: The ubiquitin-proteasome system (UPS) controls the stability, localization and/or activity of the proteome. However, the identification and characterization of complex individual ubiquitination cascades and their modulators remains a challenge. Here, we report a broadly applicable, multi...

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Detalhes bibliográficos
Publicado no:BMC Biol
Main Authors: Koszela, Joanna, Pham, Nhan T., Evans, David, Mann, Stefan, Perez-Pi, Irene, Shave, Steven, Ceccarelli, Derek F. J., Sicheri, Frank, Tyers, Mike, Auer, Manfred
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6086040/
https://ncbi.nlm.nih.gov/pubmed/30097011
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12915-018-0554-z
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