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Kinetics of duplex formation for individual DNA strands within a single protein nanopore

A single oligonucleotide was covalently attached to a genetically engineered subunit of the heptameric protein pore, α-hemolysin, to allow DNA duplex formation inside the pore lumen. Single-channel current recording was used to study the properties of the modified pore. On addition of an oligonucleo...

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Detalhes bibliográficos
Main Authors: Howorka, Stefan, Movileanu, Liviu, Braha, Orit, Bayley, Hagan
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC60813/
https://ncbi.nlm.nih.gov/pubmed/11606775
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.231434698
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