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Probing distance and electrical potential within a protein pore with tethered DNA.

DNA molecules tethered inside a protein pore can be used as a tool to probe distance and electrical potential. The approach and its limitations were tested with alpha-hemolysin, a pore of known structure. A single oligonucleotide was attached to an engineered cysteine to allow the binding of complem...

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Bibliografiske detaljer
Main Authors: Howorka, Stefan, Bayley, Hagan
Format: Artigo
Sprog:Inglês
Udgivet: 2002
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302397/
https://ncbi.nlm.nih.gov/pubmed/12496089
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