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Coevolution of both Thermostability and Activity of Polyphosphate Glucokinase from Thermobifida fusca YX

Thermostability and specific activity of enzymes are two of the most important properties for industrial biocatalysts. Here, we developed a petri dish-based double-layer high-throughput screening (HTS) strategy for rapid identification of desired mutants of polyphosphate glucokinase (PPGK) from a th...

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Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteurs principaux: Zhou, Wei, Huang, Rui, Zhu, Zhiguang, Zhang, Yi-Heng P. Job
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6070756/
https://ncbi.nlm.nih.gov/pubmed/29884753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01224-18
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