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Coevolution of both Thermostability and Activity of Polyphosphate Glucokinase from Thermobifida fusca YX

Thermostability and specific activity of enzymes are two of the most important properties for industrial biocatalysts. Here, we developed a petri dish-based double-layer high-throughput screening (HTS) strategy for rapid identification of desired mutants of polyphosphate glucokinase (PPGK) from a th...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Zhou, Wei, Huang, Rui, Zhu, Zhiguang, Zhang, Yi-Heng P. Job
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6070756/
https://ncbi.nlm.nih.gov/pubmed/29884753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01224-18
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