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A Novel CRISPR/Cas9-Based Cellular Model to Explore Adenylyl Cyclase and cAMP Signaling

Functional characterization of adenylyl cyclase (AC) isoforms has proven challenging in mammalian cells because of the endogenous expression of multiple AC isoforms and the high background cAMP levels induced by nonselective AC activators. To simplify the characterization of individual transmembrane...

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Detalhes bibliográficos
Publicado no:Mol Pharmacol
Main Authors: Soto-Velasquez, Monica, Hayes, Michael P., Alpsoy, Aktan, Dykhuizen, Emily C., Watts, Val J.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Pharmacology and Experimental Therapeutics 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6064782/
https://ncbi.nlm.nih.gov/pubmed/29950405
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/mol.118.111849
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