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A Novel CRISPR/Cas9-Based Cellular Model to Explore Adenylyl Cyclase and cAMP Signaling

Functional characterization of adenylyl cyclase (AC) isoforms has proven challenging in mammalian cells because of the endogenous expression of multiple AC isoforms and the high background cAMP levels induced by nonselective AC activators. To simplify the characterization of individual transmembrane...

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Publicat a:Mol Pharmacol
Autors principals: Soto-Velasquez, Monica, Hayes, Michael P., Alpsoy, Aktan, Dykhuizen, Emily C., Watts, Val J.
Format: Artigo
Idioma:Inglês
Publicat: The American Society for Pharmacology and Experimental Therapeutics 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6064782/
https://ncbi.nlm.nih.gov/pubmed/29950405
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/mol.118.111849
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