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Effect of linkers on immobilization of scFvs with biotin-streptavidin interaction

Single-chain variable fragment antibodies (scFvs) are attractive for use in applications that require high specificity and binding to a target, such as biosensors. Previously, we demonstrated that a variety of scFvs can be immobilized onto a streptavidin surface through in vivo biotinylation of the...

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Publicado en:Biotechnol Appl Biochem
Autores principales: Ikonomova, Svetlana P., Le, Megan T., Kalla, Neha, Karlsson, Amy J.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2018
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6064679/
https://ncbi.nlm.nih.gov/pubmed/29377386
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bab.1645
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