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Screening One Bead One Compound Libraries Against Serum Using a Flow Cytometer: Determination of the Minimum Antibody Concentration Required for Ligand Discovery
One bead one compound (OBOC) libraries can be screened against serum samples to identify ligands to antibodies in this mixture. In this protocol, hit beads are identified by staining with a fluorescent labeled secondary antibody. When screens are conducted against two different sets of serum, antibo...
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| Publicado no: | Bioorg Med Chem Lett |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6064678/ https://ncbi.nlm.nih.gov/pubmed/29395976 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2018.01.033 |
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