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Slippage of degenerate primers can cause variation in amplicon length

It is well understood that homopolymer regions should be avoided for primer binding to prevent off-target amplification. However, in metabarcoding, it is often difficult to avoid primer degeneracy in order to maximize taxa detection. We here investigate primer binding specificity using different pri...

詳細記述

保存先:
書誌詳細
出版年:Sci Rep
主要な著者: Elbrecht, Vasco, Hebert, Paul D. N., Steinke, Dirk
フォーマット: Artigo
言語:Inglês
出版事項: Nature Publishing Group UK 2018
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC6054607/
https://ncbi.nlm.nih.gov/pubmed/30030475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-29364-z
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