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Can DNA-Based Ecosystem Assessments Quantify Species Abundance? Testing Primer Bias and Biomass—Sequence Relationships with an Innovative Metabarcoding Protocol

Metabarcoding is an emerging genetic tool to rapidly assess biodiversity in ecosystems. It involves high-throughput sequencing of a standard gene from an environmental sample and comparison to a reference database. However, no consensus has emerged regarding laboratory pipelines to screen species di...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Elbrecht, Vasco, Leese, Florian
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4496048/
https://ncbi.nlm.nih.gov/pubmed/26154168
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0130324
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