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A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs
The RNA-guided nucleases derived from the CRISPR-Cas systems in bacteria and archaea have found numerous applications in biotechnology, including genome editing, imaging, and gene regulation. However, the discovery of novel Cas nucleases has outpaced their characterization and subsequent exploitatio...
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| Publicado no: | Methods |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6051895/ https://ncbi.nlm.nih.gov/pubmed/29486239 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.02.016 |
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