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A set of novel CRISPR-based integrative vectors for Saccharomyces cerevisiae

Integrating a desired DNA sequence into the yeast genomes is a widely-used genetic manipulation in the budding yeast Saccharomyces cerevisiae. The conventional integration method is to use an integrative plasmid such as pRS or YIplac series as the target DNA carrier. The nature of this method risks...

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Detalles Bibliográficos
Publicado en:Wellcome Open Res
Main Authors: Daniels, Peter W, Mukherjee, Anuradha, Goldman, Alastair SH, Hu, Bin
Formato: Artigo
Idioma:Inglês
Publicado: F1000 Research Limited 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6051238/
https://ncbi.nlm.nih.gov/pubmed/30057946
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.14642.2
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