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Post-Translational Sortase-Mediated Attachment of High-Strength Force Spectroscopy Handles

[Image: see text] Single-molecule force spectroscopy greatly benefits from site-specific surface immobilization and specific probing with a functionalized cantilever. Here, we describe a streamlined approach to such experiments by covalently attaching mechanically stable receptors onto proteins of i...

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Detalles Bibliográficos
Publicado en:ACS Omega
Main Authors: Durner, Ellis, Ott, Wolfgang, Nash, Michael A., Gaub, Hermann E.
Formato: Artigo
Idioma:Inglês
Publicado: American Chemical Society 2017
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6044863/
https://ncbi.nlm.nih.gov/pubmed/30023682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.7b00478
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