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Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers

BACKGROUND: RNA-seq and small RNA-seq are powerful, quantitative tools to study gene regulation and function. Common high-throughput sequencing methods rely on polymerase chain reaction (PCR) to expand the starting material, but not every molecule amplifies equally, causing some to be overrepresente...

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Foilsithe in:BMC Genomics
Main Authors: Fu, Yu, Wu, Pei-Hsuan, Beane, Timothy, Zamore, Phillip D., Weng, Zhiping
Formáid: Artigo
Teanga:Inglês
Foilsithe: BioMed Central 2018
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6044086/
https://ncbi.nlm.nih.gov/pubmed/30001700
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-018-4933-1
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