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WHOLE-MOUNT IMMUNOFLUORESCENCE FOR VISUALIZING ENDOGENOUS PROTEIN AND INJECTED RNA IN XENOPUS OOCYTES

The Xenopus laevis oocyte provides a unique model to study cell polarity. During oogenesis, numerous mRNAs and proteins become asymmetrically distributed in the oocyte cytoplasm and their analysis is important for understanding the establishment of developmental polarity. Microinjection of fluoresce...

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Detalhes bibliográficos
Publicado no:Cold Spring Harb Protoc
Main Authors: Jeschonek, Samantha P., Mowry, Kimberly L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6039286/
https://ncbi.nlm.nih.gov/pubmed/29321279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot097022
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