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WHOLE-MOUNT IMMUNOFLUORESCENCE FOR VISUALIZING ENDOGENOUS PROTEIN AND INJECTED RNA IN XENOPUS OOCYTES
The Xenopus laevis oocyte provides a unique model to study cell polarity. During oogenesis, numerous mRNAs and proteins become asymmetrically distributed in the oocyte cytoplasm and their analysis is important for understanding the establishment of developmental polarity. Microinjection of fluoresce...
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| Publié dans: | Cold Spring Harb Protoc |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6039286/ https://ncbi.nlm.nih.gov/pubmed/29321279 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot097022 |
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