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Visualization of mRNA Localization in Xenopus Oocytes

Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overco...

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Detalhes bibliográficos
Main Authors: Gagnon, James A., Mowry, Kimberly L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3181151/
https://ncbi.nlm.nih.gov/pubmed/21431735
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-005-8_5
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