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Visualization of mRNA Localization in Xenopus Oocytes
Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overco...
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| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2011
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3181151/ https://ncbi.nlm.nih.gov/pubmed/21431735 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-005-8_5 |
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