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Method for measuring extracellular flux from intact polarized epithelial monolayers

PURPOSE: The Seahorse XFp platform is widely used for metabolic assessment of cultured cells. Current methods require replating of cells into specialized plates. This is problematic for certain cell types, such as primary human fetal RPE (hfRPE) cells, which must be cultured for months to become pro...

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Veröffentlicht in:Mol Vis
Hauptverfasser: Calton, Melissa A., Beaulieu, Marielle O., Benchorin, Gillie, Vollrath, Douglas
Format: Artigo
Sprache:Inglês
Veröffentlicht: Molecular Vision 2018
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6031101/
https://ncbi.nlm.nih.gov/pubmed/30034209
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