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Method for measuring extracellular flux from intact polarized epithelial monolayers

PURPOSE: The Seahorse XFp platform is widely used for metabolic assessment of cultured cells. Current methods require replating of cells into specialized plates. This is problematic for certain cell types, such as primary human fetal RPE (hfRPE) cells, which must be cultured for months to become pro...

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Vydáno v:Mol Vis
Hlavní autoři: Calton, Melissa A., Beaulieu, Marielle O., Benchorin, Gillie, Vollrath, Douglas
Médium: Artigo
Jazyk:Inglês
Vydáno: Molecular Vision 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6031101/
https://ncbi.nlm.nih.gov/pubmed/30034209
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