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CAPTURE: In Situ Analysis of Chromatin Composition of Endogenous Genomic Loci by Biotinylated dCas9

Cis -regulatory elements (CREs) play a pivotal role in spatiotemporal control of tissue-specific gene expression, yet the molecular composition of the vast majority of CREs in native chromatin remains unknown. In this unit, we describe the CRISPR affinity purification in situ of regulatory elements...

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Detalhes bibliográficos
Publicado no:Curr Protoc Mol Biol
Main Authors: Liu, Xin, Zhang, Yuannyu, Chen, Yong, Li, Mushan, Shao, Zhen, Zhang, Michael Q., Xu, Jian
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6028316/
https://ncbi.nlm.nih.gov/pubmed/29927077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.64
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