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Rational Design of a Dephosphorylation-Resistant Reporter Enables Single-Cell Measurement of Tyrosine Kinase Activity
Although peptide-based reporters of protein tyrosine kinase (PTK) activity have been used to study PTK enzymology in vitro, the application of these reporters to intracellular conditions is compromised by their dephosphorylation, preventing PTK activity measurements. Non-proteinogenic amino acids ma...
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| Publicado no: | ACS Chem Biol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6024252/ https://ncbi.nlm.nih.gov/pubmed/26587880 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.5b00667 |
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