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Rational Design of a Dephosphorylation-Resistant Reporter Enables Single-Cell Measurement of Tyrosine Kinase Activity

Although peptide-based reporters of protein tyrosine kinase (PTK) activity have been used to study PTK enzymology in vitro, the application of these reporters to intracellular conditions is compromised by their dephosphorylation, preventing PTK activity measurements. Non-proteinogenic amino acids ma...

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Detalhes bibliográficos
Publicado no:ACS Chem Biol
Main Authors: Turner, Abigail H., Lebhar, Michael S., Proctor, Angela, Wang, Qunzhao, Lawrence, David S., Allbritton, Nancy L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6024252/
https://ncbi.nlm.nih.gov/pubmed/26587880
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.5b00667
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