Llwytho...

Development of a Peptidase-Resistant Substrate for Single-Cell Measurement of Protein Kinase B Activation

An iterative design strategy using three criteria was utilized to develop a peptidase-resistant substrate peptide for protein kinase B. Libraries of peptides possessing non-native amino acids were screened for time to 50% phosphorylation, degradation half-life within a lysate, and appearance of a do...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Proctor, Angela, Wang, Qunzhao, Lawrence, David S., Allbritton, Nancy L.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2012
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3428732/
https://ncbi.nlm.nih.gov/pubmed/22881604
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac301489d
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