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Development of a Peptidase-Resistant Substrate for Single-Cell Measurement of Protein Kinase B Activation
An iterative design strategy using three criteria was utilized to develop a peptidase-resistant substrate peptide for protein kinase B. Libraries of peptides possessing non-native amino acids were screened for time to 50% phosphorylation, degradation half-life within a lysate, and appearance of a do...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3428732/ https://ncbi.nlm.nih.gov/pubmed/22881604 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac301489d |
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