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Measurement of Protein Tyrosine Phosphatase Activity in Single Cells by Capillary Electrophoresis

A fluorescent peptide substrate was used to measure dephosphorylation by protein tyrosine phosphatases (PTP) in cell lysates, and single cells and to investigate the effect of environmental toxins on PTP activity in these systems. Dephosphorylation of the substrate by PTPN1 and PTPN2 obeyed Michaeli...

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Detalhes bibliográficos
Main Authors: Phillips, Ryan M., Bair, Eric, Lawrence, David S., Sims, Christopher E., Allbritton, Nancy L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3704224/
https://ncbi.nlm.nih.gov/pubmed/23682679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac401106e
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