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Development of a chemically defined platform fed-batch culture media for monoclonal antibody-producing CHO cell lines with optimized choline content
A chemically defined platform basal medium and feed media were developed using a single Chinese hamster ovary (CHO) cell line that produces a monoclonal antibody (mAb). Cell line A, which showed a peak viable cell density of 5.9 × 10(6) cells/mL and a final mAb titer of 0.5 g/L in batch culture, was...
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| Veröffentlicht in: | Cytotechnology |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Springer Netherlands
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6021290/ https://ncbi.nlm.nih.gov/pubmed/29322349 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-017-0185-1 |
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