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Development of a chemically defined platform fed-batch culture media for monoclonal antibody-producing CHO cell lines with optimized choline content

A chemically defined platform basal medium and feed media were developed using a single Chinese hamster ovary (CHO) cell line that produces a monoclonal antibody (mAb). Cell line A, which showed a peak viable cell density of 5.9 × 10(6) cells/mL and a final mAb titer of 0.5 g/L in batch culture, was...

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Detalhes bibliográficos
Publicado no:Cytotechnology
Main Authors: Kuwae, Shinobu, Miyakawa, Ichiko, Doi, Tomohiro
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Netherlands 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6021290/
https://ncbi.nlm.nih.gov/pubmed/29322349
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-017-0185-1
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