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Rapid Purification of Nuclei from Animal and Plant Tissues and Cultured Cells

Nuclei are isolated by buoyant density banding in a discontinuous iodixanol gradient, under isoosmotic conditions. The low viscosity of the gradient allows the purification to be carried out at 10,000g in only 20 min. The method avoids possible damage to nucleoprotein complexes caused by hyperosmoti...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:ScientificWorldJournal
Prif Awdur: Graham, John
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: TheScientificWorldJOURNAL 2002
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6009280/
https://ncbi.nlm.nih.gov/pubmed/12806139
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1100/tsw.2002.832
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