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Rapid Purification of Nuclei from Animal and Plant Tissues and Cultured Cells
Nuclei are isolated by buoyant density banding in a discontinuous iodixanol gradient, under isoosmotic conditions. The low viscosity of the gradient allows the purification to be carried out at 10,000g in only 20 min. The method avoids possible damage to nucleoprotein complexes caused by hyperosmoti...
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| 出版年: | ScientificWorldJournal |
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| 第一著者: | |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
TheScientificWorldJOURNAL
2002
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6009280/ https://ncbi.nlm.nih.gov/pubmed/12806139 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1100/tsw.2002.832 |
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