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Rapid Purification of Nuclei from Animal and Plant Tissues and Cultured Cells

Nuclei are isolated by buoyant density banding in a discontinuous iodixanol gradient, under isoosmotic conditions. The low viscosity of the gradient allows the purification to be carried out at 10,000g in only 20 min. The method avoids possible damage to nucleoprotein complexes caused by hyperosmoti...

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Bibliografiske detaljer
Udgivet i:ScientificWorldJournal
Hovedforfatter: Graham, John
Format: Artigo
Sprog:Inglês
Udgivet: TheScientificWorldJOURNAL 2002
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6009280/
https://ncbi.nlm.nih.gov/pubmed/12806139
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1100/tsw.2002.832
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