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Purification of Lipid Rafts from Cultured Cells

Lipid-rich lipid rafts are microdomains of the plasma membrane that are resistant to low concentrations of nonionic detergent. This forms the basis for their isolation. Either a microsomal fraction or a postnuclear supernatant are loaded beneath a discontinuous iodixanol gradient. If all the solutio...

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Detalles Bibliográficos
Publicado en:ScientificWorldJournal
Autor Principal: Graham, John
Formato: Artigo
Idioma:Inglês
Publicado: TheScientificWorldJOURNAL 2002
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6009391/
https://ncbi.nlm.nih.gov/pubmed/12806159
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1100/tsw.2002.846
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