Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2,4-dinitrophenylhydrazine-based photometric assay

A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by protein carbonyl-DNPH hydrazone formation at acidic pH in the presence of denaturing urea, and subsequent hydrazone solubilization in the...

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Detalhes bibliográficos
Publicado no:Redox Biol
Main Authors: Georgiou, Christos D., Zisimopoulos, Dimitrios, Argyropoulou, Vasiliki, Kalaitzopoulou, Electra, Salachas, George, Grune, Tilman
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6006683/
https://ncbi.nlm.nih.gov/pubmed/29684819
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.redox.2018.04.010
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