Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay

A new fluorometric assay is presented for the ultrasensitive quantification of total protein carbonyls, and is based on their specific reaction with rhodamine B hydrazide (RBH), and the production of a protein carbonyl-RBH hydrazone the fluorescence of which (at ex/em 560/585 nm) is greatly enhanced...

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Detalhes bibliográficos
Publicado no:Redox Biol
Main Authors: Georgiou, Christos D., Zisimopoulos, Dimitrios, Argyropoulou, Vasiliki, Kalaitzopoulou, Electra, Ioannou, Panayiotis V., Salachas, George, Grune, Tilman
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6006725/
https://ncbi.nlm.nih.gov/pubmed/29727801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.redox.2018.04.017
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