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Precise Editing at DNA Replication Forks Enables Multiplex Genome Engineering in Eukaryotes
We describe a multiplex genome engineering technology in Saccharomyces cerevisiae based on annealing of synthetic oligonucleotides at the lagging strand of DNA replication. The mechanism is independent of Rad51-directed homologous recombination and avoids the creation of double-strand DNA breaks, en...
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| Publicado no: | Cell |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5995112/ https://ncbi.nlm.nih.gov/pubmed/29153834 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2017.10.034 |
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