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Precise Editing at DNA Replication Forks Enables Multiplex Genome Engineering in Eukaryotes

We describe a multiplex genome engineering technology in Saccharomyces cerevisiae based on annealing of synthetic oligonucleotides at the lagging strand of DNA replication. The mechanism is independent of Rad51-directed homologous recombination and avoids the creation of double-strand DNA breaks, en...

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Bibliografske podrobnosti
izdano v:Cell
Main Authors: Barbieri, Edward M., Muir, Paul, Akhuetie-Oni, Benjamin O., Yellman, Christopher M., Isaacs, Farren J.
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5995112/
https://ncbi.nlm.nih.gov/pubmed/29153834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2017.10.034
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