Cargando...

Precise Editing at DNA Replication Forks Enables Multiplex Genome Engineering in Eukaryotes

We describe a multiplex genome engineering technology in Saccharomyces cerevisiae based on annealing of synthetic oligonucleotides at the lagging strand of DNA replication. The mechanism is independent of Rad51-directed homologous recombination and avoids the creation of double-strand DNA breaks, en...

Descrición completa

Gardado en:
Detalles Bibliográficos
Publicado en:Cell
Main Authors: Barbieri, Edward M., Muir, Paul, Akhuetie-Oni, Benjamin O., Yellman, Christopher M., Isaacs, Farren J.
Formato: Artigo
Idioma:Inglês
Publicado: 2017
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5995112/
https://ncbi.nlm.nih.gov/pubmed/29153834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2017.10.034
Tags: Engadir etiqueta
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!