SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology

We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gen...

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Detalhes bibliográficos
Publicado no:Genome Biol
Main Authors: Abdelmoez, Mahmoud N., Iida, Kei, Oguchi, Yusuke, Nishikii, Hidekazu, Yokokawa, Ryuji, Kotera, Hidetoshi, Uemura, Sotaro, Santiago, Juan G., Shintaku, Hirofumi
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5989370/
https://ncbi.nlm.nih.gov/pubmed/29871653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-018-1446-9
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