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Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution
BACKGROUND: Mesophilic alkaline serine proteases from various bacteria have been commercially applied in a range of industries owing to their high catalytic efficiency and wide substrate specificity. However, these proteases have an optimal catalytic temperature of approximately 50 °C, and their act...
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| Publicado no: | BMC Biotechnol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5984802/ https://ncbi.nlm.nih.gov/pubmed/29859069 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-018-0451-0 |
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