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Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution

BACKGROUND: Mesophilic alkaline serine proteases from various bacteria have been commercially applied in a range of industries owing to their high catalytic efficiency and wide substrate specificity. However, these proteases have an optimal catalytic temperature of approximately 50 °C, and their act...

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Bibliografske podrobnosti
izdano v:BMC Biotechnol
Main Authors: Zhao, Hong-Yan, Feng, Hong
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5984802/
https://ncbi.nlm.nih.gov/pubmed/29859069
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-018-0451-0
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