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A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast
The CRISPR/Cas9 system, which relies on RNA‐guided DNA cleavage to induce site-specific DNA double-strand breaks, is a powerful tool for genome editing. This system has been successfully adapted for the fission yeast Schizosaccharomyces pombe by expressing Cas9 and the single-guide RNA (sgRNA) from...
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| Veröffentlicht in: | G3 (Bethesda) |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Genetics Society of America
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5982833/ https://ncbi.nlm.nih.gov/pubmed/29703785 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.118.200164 |
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