Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System

CRISPR-Cas systems offer versatile technologies for genome engineering, yet their implementation has been outpaced by ongoing discoveries of new Cas nucleases and anti-CRISPR proteins. Here, we present the use of E. coli cell-free transcription-translation (TXTL) systems to vastly improve the speed...

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Pubblicato in:Mol Cell
Autori principali: Marshall, Ryan, Maxwell, Colin S., Collins, Scott P., Jacobsen, Thomas, Luo, Michelle L., Begemann, Matthew B., Gray, Benjamin N., January, Emma, Singer, Anna, He, Yonghua, Beisel, Chase L., Noireaux, Vincent
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2018
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5976856/
https://ncbi.nlm.nih.gov/pubmed/29304331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2017.12.007
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