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Short DNA containing χ sites enhances DNA stability and gene expression in E. coli cell-free transcription-translation systems

E. coli cell-free transcription-translation (TXTL) systems offer versatile platforms for advanced biomanufacturing and for prototyping synthetic biological parts and devices. Production and testing could be accelerated with the use of linear DNA, which can be rapidly and cheaply synthesized. However...

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Detalhes bibliográficos
Publicado no:Biotechnol Bioeng
Main Authors: Marshall, Ryan, Maxwell, Colin S., Collins, Scott P., Beisel, Chase L., Noireaux, Vincent
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5522353/
https://ncbi.nlm.nih.gov/pubmed/28475211
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.26333
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