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A Cleavage-Responsive Stem-Loop Hairpin for Assaying Guide RNA Activity
The scope of the CRISPR-Cas9 technology now reaches far beyond genomic engineering. While significant efforts are driving the evolution of this revolutionary biomedical tool, the in vitro cleavage assay remains the standard method implemented to validate the guide RNA that directs endonuclease Cas9...
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| Publicado no: | ACS Chem Biol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5966300/ https://ncbi.nlm.nih.gov/pubmed/29381046 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.7b00899 |
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