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Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets

Poly (ADP-ribose)ylation is a dynamic protein modification that regulates multiple cellular processes. Here, we describe a system for identifying and characterizing PARylation events that exploits the ability of a PBZ (PAR-binding zinc finger) protein domain to bind PAR with high-affinity. By linkin...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Krastev, Dragomir B., Pettitt, Stephen J., Campbell, James, Song, Feifei, Tanos, Barbara E., Stoynov, Stoyno S., Ashworth, Alan, Lord, Christopher J.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5964205/
https://ncbi.nlm.nih.gov/pubmed/29789535
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-04466-4
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