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Expressed repetitive elements are broadly applicable reference targets for normalization of reverse transcription-qPCR data in mice

Reverse transcription quantitative PCR (RT-qPCR) is the gold standard method for gene expression analysis on mRNA level. To remove experimental variation, expression levels of the gene of interest are typically normalized to the expression level of stably expressed endogenous reference genes. Identi...

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Publicat a:Sci Rep
Autors principals: Renard, Marjolijn, Vanhauwaert, Suzanne, Vanhomwegen, Marine, Rihani, Ali, Vandamme, Niels, Goossens, Steven, Berx, Geert, Van Vlierberghe, Pieter, Haigh, Jody J., Decaesteker, Bieke, Van Laere, Jolien, Lambertz, Irina, Speleman, Frank, Vandesompele, Jo, Willaert, Andy
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5955877/
https://ncbi.nlm.nih.gov/pubmed/29769563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-25389-6
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