Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Gene engineering in mouse embryos or embryonic stem cells (mESCs) allows for the study of the function of a given protein. Proteins are the workhorses of the cell and often consist of multiple functional domains, which can be influenced by posttranslational modifications. The depletion of the entire...

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Bibliografische gegevens
Gepubliceerd in:J Vis Exp
Hoofdauteurs: Pieters, Tim, Haenebalcke, Lieven, Bruneel, Kenneth, Vandamme, Niels, Hochepied, Tino, van Hengel, Jolanda, Wirth, Dagmar, Berx, Geert, Haigh, Jody J., van Roy, Frans, Goossens, Steven
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MyJove Corporation 2017
Onderwerpen:
Developmental Biology
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5565114/
https://ncbi.nlm.nih.gov/pubmed/28518103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55575
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